clustalx（JBC杂志最多)）

爱科学爱自然：对于全球最高引的前100篇文章，篇均引用量是34834.92次；这100篇文章主要聚集在生物化学及分子生物学领域；这100篇文章中，发表在PHYSICAL REVIEW B杂志及JBC杂志都是发表了7篇文章；对于这100篇文章，产出率最高的机构是加州大学；总的来说，这100篇文章主要是聚集在基础研究，如蛋白质的定量，蛋白质的分析，实时定量PCR技术，BLAST的诞生，DNA测序，同源序列比对等方面。iNature编辑组的统计时间是2017年11月26日。

1.引用量最高的10000篇文章分析

我们以Web of Science为基础，通过相关的检索，从1874-2018年，总共检索到120611782条记录（图.1），之后我们根据引用量从高到底，进行排序，我们筛选了10000条记录（因为Web of Science最多只能一次分析10000条记录），进行相应的统计分析。

图.1 总文章数

我们筛选了引用量排名前10000的文章，发现每篇文章的平均引用次数是3173.14次，总引用次数是31731393次，施引文献是14692585 （图.2）。另外，我们发现，最高引用文章大部分都聚集在1990-2010年之间，达到了40%以上。

图.2 10000篇文章引用分析

之后，我们看了一下不同时间段，对于这些文章的引用次数分析，发现绝大部分引用都是聚集在1990-2016年之间（图.3）。

图.3 10000篇文章不同时间段引用分析

由于分析10000篇文章，工作量太大，我们就直接分析前100篇文章，发现前100篇文章的平均引用量是34834.92次，总引用次数是3483492次，施引文献达到2627346篇。这100篇文章，占前10000篇的总引用的10.98%，但是总文章数是1.00%，故我们主要分析这100篇文章（图.4）。

图.4 100篇文章引用分析

2.引用量最高的100篇文章分析

对于这100文章发表的时间进行分析，我们发现在1950年以前只有4篇，1950-1960年之间，有10篇；1961-1970年，有9篇；1971-2010年，总共有74篇，占了绝大部分；2010年以后，只有3篇文章（图.5），这三篇文章分别是遗传进化（MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods，2011年），癌症综述（Hallmarks of Cancer: The Next Generation，2011年），全球癌症统计（Global Cancer Statistics，2011年）。

图.5 100篇文章发表时间分析

其次我们对这100篇文章的种类进行了基本分析，发现生物化学及分子生物学方面有35篇，物理学有15篇，化学有14篇，数学11篇（图.6）。

图.6 100篇文章方向分析

我们再次对这100篇文章发表的杂志进行分析（>3篇），发表在PHYSICAL REVIEW B杂志及JBC杂志都是7篇文章；ANALYTICAL BIOCHEMISTRY，JOURNAL OF CHEMICAL PHYSICS，NATURE，NUCLEIC ACIDS RESEARCH都是发表了4篇文章，总的来说，这高引用的100篇文章，大部分都是生物，物理，生物化学及化学方面的文章。另外这10个杂志占了39篇文章（图.7）。

图.7 100篇文章杂志分析

我们再次统计了国家，发现美国有44篇，占了绝大部分，其次是英国，达到9篇；德国有6篇，很遗憾，没有发现有中国参与的文章（图.8）。

图.8 100篇文章国家分布

我们对研究机构统计了一下，发现加州大学系统占的比例最大，达到了7篇，其次是宾夕法尼亚大学，达到了4篇（图.9）。

图.9 100篇文章的大学或研究所分析

3.引用量最高的100篇文章列表

对于这100篇文章，主要是技术的变革及基础研究，如蛋白质的定量，蛋白质的分析，实时定量PCR技术，BLAST的诞生，DNA测序，同源序列比对等，这些东西都是非常的基础，故这也注定了它们的高引用量（图.10）。

标题总引用次数年均引用1PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT3358445012.62CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T42447855099.693RAPID AND SENSITIVE METHOD FOR QUANTITATION OF MICROGRAM QUANTITIES OF PROTEIN UTILIZING PRINCIPLE OF PROTEIN-DYE BINDING2013134793.174DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS667901629.025DENSITY-FUNCTIONAL THERMOCHEMISTRY .3. THE ROLE OF EXACT EXCHANGE652442609.766Generalized gradient approximation made simple634842885.647SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION631612037.458DEVELOPMENT OF THE COLLE-SALVETTI CORRELATION-ENERGY FORMULA INTO A FUNCTIONAL OF THE ELECTRON-DENSITY614062046.879A short history of SHELX58640586410Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method581593421.1211ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS551211413.3612A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES51444843.3413BASIC LOCAL ALIGNMENT SEARCH TOOL491161754.1414CLUSTAL-W - IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE474441976.8315Gapped BLAST and PSI-BLAST: a new generation of protein database search programs455572169.3816NONPARAMETRIC-ESTIMATION FROM INCOMPLETE OBSERVATIONS44604743.417MINI-MENTAL STATE - PRACTICAL METHOD FOR GRADING COGNITIVE STATE OF PATIENTS FOR CLINICIAN436371014.8118A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES41340738.2119THE NEIGHBOR-JOINING METHOD - A NEW METHOD FOR RECONSTRUCTING PHYLOGENETIC TREES387351249.5220A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION37927642.8321REVISED EFFECTIVE IONIC-RADII AND SYSTEMATIC STUDIES OF INTERATOMIC DISTANCES IN HALIDES AND CHALCOGENIDES37143884.3622DENSITY-FUNCTIONAL EXCHANGE-ENERGY APPROXIMATION WITH CORRECT ASYMPTOTIC-BEHAVIOR340651135.523SELF-CONSISTENT EQUATIONS INCLUDING EXCHANGE AND CORRELATION EFFECTS33872639.0924Efficient iterative schemes for ab initio total-energy calculations using a plane-wave basis set336581529.9125The moderator-mediator variable distinction in social psychological research: conceptual, strategic, and statistical considerations.331911037.2226Processing of X-ray diffraction data collected in oscillation mode326671555.5727The status, quality, and expansion of the NIH full-length cDNA project: The Mammalian Gene Collection (MGC)323912313.6428DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY GEL-ELECTROPHORESIS32239749.7429REGRESSION MODELS AND LIFE-TABLES32022696.1330COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES31882514.2331RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS31292894.0632The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools307701465.2433HELICAL MICROTUBULES OF GRAPHITIC CARBON303861125.4134STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT29847932.7235FUZZY SETS29740561.1336CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING296911290.9137Electric field effect in atomically thin carbon films285202037.1438INHOMOGENEOUS ELECTRON GAS2797251839CONFIDENCE-LIMITS ON PHYLOGENIES - AN APPROACH USING THE BOOTSTRAP27721840.0340SPECIAL POINTS FOR BRILLOUIN-ZONE INTEGRATIONS27045643.9341MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods265813797.2942USE OF LEAD CITRATE AT HIGH PH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY25405461.9143From ultrasoft pseudopotentials to the projector augmented-wave method250571318.7944MEASUREMENT OF OBSERVER AGREEMENT FOR CATEGORICAL DATA25005609.8845PROJECTOR AUGMENTED-WAVE METHOD243141013.0846MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0240662187.8247RELIABILITY OF MOLECULAR WEIGHT DETERMINATIONS BY DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS23835486.4348THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS23763344.3949ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE23574604.4650The colorimetric determination of phosphorus23420251.8351Cutoff Criteria for Fit Indexes in Covariance Structure Analysis: Conventional Criteria Versus New Alternatives227551197.6352Particle swarm optimization2258698253DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS22463415.9854MAXIMUM LIKELIHOOD FROM INCOMPLETE DATA VIA EM ALGORITHM22259542.955A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY21557615.9156Efficiency of ab-initio total energy calculations for metals and semiconductors using a plane-wave basis set21539979.0557NEW LOOK AT STATISTICAL-MODEL IDENTIFICATION21172481.1858Global Cancer Statistics209832997.5759A NEW GENERATION OF CA-2+ INDICATORS WITH GREATLY IMPROVED FLUORESCENCE PROPERTIES20677626.5860CLINICAL-DIAGNOSIS OF ALZHEIMERS-DISEASE - REPORT OF THE NINCDS-ADRDA WORK GROUP UNDER THE AUSPICES OF DEPARTMENT-OF-HEALTH-AND-HUMAN-SERVICES TASK-FORCE ON ALZHEIMERS-DISEASE20596605.7661THE ASSESSMENT AND ANALYSIS OF HANDEDNESS: THE EDINBURGH INVENTORY20514436.4762The rise of graphene205051864.0963Distinctive image features from scale-invariant keypoints20258144764A RATING SCALE FOR DEPRESSION20238348.9365AN INVENTORY FOR MEASURING DEPRESSION20104352.766EQUATION OF STATE CALCULATIONS BY FAST COMPUTING MACHINES19396298.467ESTIMATION OF CONCENTRATION OF LOW-DENSITY LIPOPROTEIN CHOLESTEROL IN PLASMA, WITHOUT USE OF PREPARATIVE ULTRACENTRIFUGE19380421.368HIGH-RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS OF PROTEINS19179446.0269COMPARISON OF SIMPLE POTENTIAL FUNCTIONS FOR SIMULATING LIQUID WATER19023543.5170THE MOS 36-ITEM SHORT-FORM HEALTH SURVEY (SF-36) .1. CONCEPTUAL-FRAMEWORK AND ITEM SELECTION18987730.2771MicroRNAs: Genomics, biogenesis, mechanism, and function188451346.0772PHASE ANNEALING IN SHELX-90 - DIRECT METHODS FOR LARGER STRUCTURES18787670.9673A LOW-COST, HIGH-EFFICIENCY SOLAR-CELL BASED ON DYE-SENSITIZED COLLOIDAL TIO2 FILMS18733693.8174GAUSSIAN-BASIS SETS FOR USE IN CORRELATED MOLECULAR CALCULATIONS .1. THE ATOMS BORON THROUGH NEON AND HYDROGEN1853163975OPTIMIZATION BY SIMULATED ANNEALING18374524.9776A NEW METHOD OF CLASSIFYING PROGNOSTIC CO-MORBIDITY IN LONGITUDINAL-STUDIES - DEVELOPMENT AND VALIDATION18044582.0677MrBayes 3: Bayesian phylogenetic inference under mixed models179941199.678TISSUE SULFHYDRYL GROUPS17989304.979VMD: Visual molecular dynamics17956816.1880METAANALYSIS IN CLINICAL-TRIALS17945560.7881IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES17744479.5782HOMEOSTASIS MODEL ASSESSMENT - INSULIN RESISTANCE AND BETA-CELL FUNCTION FROM FASTING PLASMA-GLUCOSE AND INSULIN CONCENTRATIONS IN MAN17634534.3683A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN17621489.4784Measuring inconsistency in meta-analyses175841172.2785MEASUREMENT OF PROTEIN USING BICINCHONINIC ACID17573532.5286THE HOSPITAL ANXIETY AND DEPRESSION SCALE17458498.887STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID17232277.9488The Protein Data Bank17152952.8989The NCEP/NCAR 40-year reanalysis project17118778.0990Collective dynamics of 'small-world' networks17044852.291Hallmarks of Cancer: The Next Generation169132416.1492ABINITIO MOLECULAR-DYNAMICS FOR LIQUID-METALS16788671.5293Bias in meta-analysis detected by a simple, graphical test16785799.2994DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION16754242.8195A MATHEMATICAL THEORY OF COMMUNICATION16715238.7996PROCHECK - A PROGRAM TO CHECK THE STEREOCHEMICAL QUALITY OF PROTEIN STRUCTURES16710668.497MODELTEST: testing the model of DNA substitution16619830.9598MULTIPLE RANGE AND MULTIPLE F TESTS16580263.1799ESTIMATING DIMENSION OF A MODEL16551413.78100A SIMPLE METHOD FOR ESTIMATING EVOLUTIONARY RATES OF BASE SUBSTITUTIONS THROUGH COMPARATIVE STUDIES OF NUCLEOTIDE-SEQUENCES16492434

图.10 前100篇文章列表

注：所有的数据来源为Web of science。